use PS to clean up the map, using the clone tool to delete the already made route. and save it.
Then reopen the map and draw ur route.
when complete, save as map with route as opposed to the last one which can be "clean map"
In premiere over lay the routed version on top of the plain, then use which ever method ur happy with to reveal the line, such as a transition wipe left to right would work depending on if the route was just left to right. A custom transition, or even a custom matt overlay.
hope that helps.
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